Sampaguita Pictures was a Philippine film production company. It was named for the Philippine national flower, sampaguita. Though no longer functioning, the company's Sampaguita Compound remains in Quezon City. 
Sampaguita Pictures was established in the last quarter of 1937. Its first feature-length film was Bituing Marikit, starring Elsa Oria and Rogelio dela Rosa. It was a box-office hit. The company continued to make films in the action, musical, horror and suspense genres.
Before World War II began, and the Japanese occupied the Philippines, several Sampaguita films enjoyed box office success in the region. Some of them employed the famous tandem of Carmen Rosales and Rogelio dela Rosa, like Panambitan, Pagsuyo, Jazmin and many more. But the first film made at the Sampaguita studio by Carmen and Rogelio was Takipsilim.
After the war ended and the Philippines declares its independence from the United States, Sampaguita made several war pictures in 1946, including Guerilyera, with Carmen and newcomer Celso Baltazar, Maynila, the comeback picture of Corazon Noble featuring Tita Duran, and Isumpa mo Giliw, with husband-and-wife team Angel Esmeralda and Corazon Noble.
After several war musicals became popular, the studio created a new star team with real husband and wife Pancho Magalona and Tita Duran. They made several hits including Bulaklak na Walang Pangalan, Huwag Ka ng Magtampo, Sa Isang Sulyap mo Tita, and Sa Isang Halik mo Pancho.
In 1950, the studios were hit by fire and hundreds of film prints were destroyed. But in 1951, they top-billed the unknown Tessie Agana, the daughter of Linda Estrella, in her first lead role in Roberta, with another child actor Boy Alano and the movie was one of the biggest hits of the year.
In the early fifties several actors appeared in the studio's productions, including Alicia Vergel and her husband César Ramírez in Ukkala, Diwani, the Philippine folk hero Bernardo Carpio, MN and many more.
In 1952 the half-Filipino half-American Gloria Romero made her first full-length movie as the partner of Cesar Ramirez in an action costume picture Palasig.
Another famous husband-and-wife team were Rita Gomez and Ric Rodrigo. They were popular together in their movie Diyosa.
The infamous tandem of husband-and-wife Lolita Rodriguez and Eddie Arenas made some dramas together, like Kundiman ng Puso, Busabos, Cicatriz and Mapait na Lihim. The two later divorced. Arenas is still active in Philippine cinema while Rodriguez moved to the United States.
Susan Roces took her first lead role as a boxer in Sabungera opposite Luis Gonzales, but the movie was not a box office hit. Fuentes and Roces are famous for their rivalry when they made the movie Cover Girls in the 1960s.
By that time, a new manager, Dr. Jose R. Perez, took over the reins of Sampaguita.
In 1960, Sampaguita made Pitong Pagsisi supported by 20 Sampaguita stars, including the return of Paraluman who reigned in the studio in the late 40s.
In 1970s, the studio continued producing movies albeit on a limited production, especially with the Nora Aunor-Tirso Cruz III tandem culminating in the 1971 movie Guy and Pip. In 1982, Sampaguita Pictures stopped releasing movies (together with its rival LVN Pictures) after its last successful movie Batch '81. However, its post-production facilities remained in operation until 2005.
In 1995, the company ventured into television with GMA Network; they produced the drama anthology series Pira-pirasong Pangarap which lasted until 2003, which started another co-production Nagmamahal, Manay Gina. Both series were hosted by Gina de Venecia.
Unitel Pictures currently owns Sampaguita's film library. All of the films are currently archived and handled by the ABS-CBN Film Archives and the Film Development Council of the Philippines' National Film Archives of the Philippines.
- De Ocampo, Nick (November 9, 2017). Film: American Influences on Philippine Cinema. Anvil Publishing. ISBN 9789712728969.
- De Ocampo, Nick (November 9, 2017). EIGA: Cinema in the Philippines During World War II. Anvil Publishing. ISBN 9786214200832.
- Bulacan pays tribute to Dr. Perez for golden age of Philippine cinema (November 10, 2016).
- Dr. Jose Perez: The starmaker is an ideal dad (June 16, 2001).
- New Manila, Quezon City: Sampaguita Studios & the Vera-Perez Ancestral Home (February 22, 2019).
- "Sampaguita movies now in National Archives", June 28, 2011.